Nuclear Magnetic Resonance Analysis Methods

Nuclear magnetic resonance (NMR) refers to the phenomenon where the energy level of nuclei with spin split under the action of an external magnetic field, and energy level transitions can occur with a specific radio frequency radiation. NMR spectroscopy can be defined as an indispensable tool that applies a magnetic field to an atomic nucleus (e.g., the most common stable isotopes ¹H, ¹³C, ¹⁵N) and radio frequency pulses to characterize the resonant frequency of that nucleus according to its chemical or environmental surroundings. Since the independent discovery of NMR by Felix Bloch and Edward Mills Purcell in 1946, NMR techniques have been rapidly developing from both instrumental and experimental design perspectives for both solids and liquids applications. When the initial papers on the subject appeared, NMR was also considered a quantitative technique. After the 1970s, the commercialization of pulsed Fourier transform NMR spectroscopy, advances in high-field magnets, pulse trains, and cryo probe techniques have led to significant improvements in the sensitivity and resolution of NMR quantitative methods. Currently, NMR spectroscopy has made leaps and bounds and has been widely used in various disciplines in academia and industry.

As a professional testing and analysis organization, Alfa Chemistry has an experienced technical team that employs advanced analysis methods to meet the full range of our clients' NMR needs.

Our Analysis Methods

The analysis methods used at the Alfa Chemistry NMR Center include, but are not limited to the following.

Quantitative Nuclear Magnetic Resonance (qNMR) Spectroscopy

qNMR spectroscopy is an analytical method based on the principles of NMR spectroscopy. It covers a variety of nuclei, such as ¹H, ¹³C, ¹⁹F, ¹⁷O, ³¹P, etc.

Solid-state Nuclear Magnetic Resonance (NMR) Spectroscopy

Solid-state NMR spectroscopy is an atomic-level method to determine the chemical structure, 3D structure and dynamics of solids and semi-solids.

Solution-state Nuclear Magnetic Resonance (NMR) Spectroscopy

Solution-state nuclear magnetic resonance (NMR) spectroscopy is the classical NMR spectroscopy approach used for molecular structure determination, where the experimental sample is studied while in solution.

Dynamic Nuclear Polarization (DNP) NMR

Dynamic Nuclear Polarization (DNP) is an advanced hyperpolarization technique that dramatically enhances the sensitivity of solid-state NMR (ssNMR) spectroscopy.

1D NMR Spectroscopy

One-Dimensional Nuclear Magnetic Resonance (1D NMR) spectroscopy is a powerful, non-destructive analytical technique that probes the magnetic properties of atomic nuclei to reveal detailed information about molecular structure, stereochemistry, and composition.

Multidimensional NMR (2D, 3D)

Multidimensional NMR spectroscopy expands traditional nuclear magnetic resonance into two, three, or higher-frequency axes, dramatically increasing spectral resolution and resolving congested signals.

Diffusion-Ordered NMR Spectroscopy

Diffusion-Ordered NMR Spectroscopy (DOSY) is an advanced two-dimensional spectroscopic technique that correlates nuclear magnetic resonance (NMR) chemical shifts with translational diffusion coefficients.

GPC-NMR Hyphenated Platform

GPC-NMR spectroscopy is a powerful hyphenated analytical technique designed to analyze complex macromolecular systems on a fraction-by-fraction basis in real time.

HPLC-MS-SPE-NMR Hyphenated Platform

HPLC-MS-SPE-NMR hyphenation combines the separation power of liquid chromatography with the molecular weight screening of mass spectrometry and the absolute structural elucidation capability of high-field nuclear magnetic resonance.

Our services are for research use only.