Analysis of Proteins

Reliable analysis of protein molecular weight in solution is crucial for biomolecule research. Molecular weight analysis informs researchers if the correct protein has been produced and if it is suitable for use in further experiments. Protein quality control must characterize not only the purity of the final product, but also its oligomeric state, homogeneity, identity, conformation, structure, post-translational modifications and other properties. Some of the most widespread methods for analyzing protein molecular weight are sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), capillary electrophoresis (CE), native PAGE, mass spectrometry (MS), gel permeation chromatography (GPC) and analytical ultracentrifugation (AUC)^[1].

Among them, GPC is a very effective and relatively stable, simple protein analysis method that can be used to obtain high-resolution separations due to mild separation conditions, allowing true size analysis of protein samples.

Our Services

State-of-the-art instrumentation, combined with the knowledge, experience and skills of our analysts, enables us advanced materials characterization capabilities. The use of GPC to size profiling the proteins, for example, when you need to:

Our Technology Platforms

Our technology platforms include but are not limited to the following.

• Gel Permeation Chromatography - Multi-Angle Static Light Scattering (GPC-MALS)

GPC-MALS is a versatile and reliable method for characterizing solutions of one or more protein species, including monomers, native oligomers or aggregates, and heterocomplexes. Since measurements are made in each elution volume, GPC-MALS can determine whether the elution peak is homogeneous or non-uniform and distinguish between fixed molecular weight distribution and dynamic equilibrium. Modified proteins, such as glycoproteins or lipoproteins, or conjugates, such as detergent-solubilized membrane proteins, can also be analyzed.

• Gel permeation chromatography - Mass Spectrometry (GPC-MS)

Factors to Consider

When selecting an GPC column for protein analysis, there are many factors should be considered, including:

*1一 In general, smaller particles and narrower particle size distributions usually result in the higher resolution.
*2一 Molecules within the fractionation range can be separated by high-resolution fractionation.
*3一 If you set a lower flow rate, the resolution between protein peaks is higher.
*4一 For higher resolution, short and narrow capillaries should be used.

Our Advantages

• We have a leading technology platform and a team of experienced experts.
• We provide customers with the most comprehensive services at the most favorable prices to help them save scientific research funds reasonably.
• For the content entrusted by the customer, we will strictly keep the secret and sign a confidentiality agreement.
• Even for the most complex samples, we strive to provide satisfactory results for our customers.

Alfa Chemistry is committed to providing customers with high-quality GPC services to monitor protein prep quality, evaluate protein stability, study complex formation, and more. Alfa Chemistry looks forward to cooperating with you.

Reference

1. Some, D., Amartely, H., Tsadok, A., Lebendiker, M. Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS). J. Vis. Exp. 2019, (148), e59615.

Our services are for research use only.