Analysis of Biomolecules

Each cell in an organism is composed of many macromolecules (called "biomolecules") that are necessary for various cellular functions and metabolism. The biological activity of biomolecules is controlled by their interactions with other molecules within the cell. This interaction is in turn controlled by their three-dimensional (3D) structure in space [1]. Therefore, understanding the structural and kinetic information of biomolecules is crucial for a comprehensive understanding of their functions. Nuclear magnetic resonance (NMR) spectroscopy is arguably the most versatile analytical method for the study of matter. It is an indispensable analytical technique that has been successfully applied to study the structure, dynamics and interactions of biologically significant macromolecules and their complexes in the solution state. Up to now, the field of biomolecular NMR has progressed rapidly, with new advances being made every year.

Alfa Chemistry has a one-stop NMR analysis platform and has the ability to provide high-quality NMR analysis services for biomolecules. Our experienced experts are equipped with the latest technology and cutting-edge equipment to provide you with accurate and reliable information on biomolecules. Whether you need to analyze proteins, nucleic acids, or other biomolecules, Alfa Chemistry can help you achieve your research goals.

Our Services

The NMR analysis services we can provide for biomolecules include, but are not limited to the following.

(i) Study of 3D structure of biomolecules (lipids, DNA, RNA, carbohydrates, peptides / proteins, oligosaccharides)

(ii) Conformational dynamics and mobility studies of biomolecules in solution

Rotational correlation time τc, internal motion, order parameter S2
Mobility of peptide backbone in proteins
Populations of amino acid side chain conformations
Proline cis/trans isomerization
Aromatic ring flips
Sugar puckering, base pair dynamics in DNA, RNA

(iii) Chemical exchange and conformational exchange studies

Hydrogen exchange rates → hydrogen bonding
Base pair opening dynamics in DNA, RNA

(iv) Study of enzyme mechanism and chemical reactions

(v) Partially structured biomolecules → folding studies

Our Analysis Methods

The NMR methods used in our laboratory for the analysis of biomolecules include, but are not limited to the following.

Solution-state NMR Spectroscopy

Solution-state NMR Spectroscopy

In structural biology, solution-state NMR plays a central role not only in elucidating the 3D structures of biomolecules such as proteins, nucleic acids and their complexes, but also in describing how these molecules fluctuate around their equilibrium positions and how they change over time under nonequilibrium conditions ^[2].

Solid-state NMR Spectroscopy

Solid-state NMR is suitable for the analysis of samples that cannot be studied in solution, such as membrane proteins or amyloid fibrils. In addition to being used to study the structure of these samples, solid-state NMR spectroscopy is also used to gain information about dynamical aspects of biomolecules.

Solid-state NMR Spectroscopy

Our Advantages

We have a leading technology platform and a team of experienced experts.
We provide customers with the most comprehensive services at the most favorable prices to help them save scientific research funds reasonably.
For the content entrusted by the customer, we will strictly keep the secret and sign a confidentiality agreement.
Even for the most complex samples, we strive to provide satisfactory results for our customers.

Alfa Chemistry's services are widely recognized for their precision, efficiency and reliability, and are trusted by researchers and institutions worldwide. Please do not hesitate to start your next project with us.

References

Atreya, H. S. Structures of biomolecules by NMR spectroscopy. Resonance. 2015, 20: 1033-1039.
Schanda, P.; Ernst, M. Studying dynamics by magic-angle spinning solid-state NMR spectroscopy: Principles and applications to biomolecules. Progress in nuclear magnetic resonance spectroscopy. 2016, 96: 1-46.

Our services are for research use only.